Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures

Eosin Nigrosin staining for assessment of sperm vitality is an essential component basic semen analysis as it helps differentiate between dead and immotile sperms, has clinical implications in terms patient treatment follow up. This technique involves minimal use reagents simple procedural steps. Standardization the same pertinent to warrant accurate reproducible results medical laboratories, even those not specialized infertility care. We wish share our hands on experience through various stages implementing this from challenges faced putting quality control processes place reference peers.